EuroFlow PIDOT 2025
Resources
Detailed information
EuroFlow SOPs and relevant documents
EuroFlow SOP for sample preparation. Version 1.7 - November 2023
EuroFlow SOP for bulk lysis in MRD panels. Version 1.3 - June 2018
Composition and technical info of EuroFlow panels for PID. Version 1.2 - September 2022
EuroFlow SOP for instrument set-up and compensation for DxFlex. Version 1.2 - July 2023
EuroFlow SOP for instrument set-up and compensation for BD LSR II and BD FACS Canto. Version 1.2.1 - October 2019
EuroFlow SOP for instrument set-up and compensation for BD Lyric for 8-color panels. Version 1.8 - July 2019
EuroFlow SOP for instrument set-up and compensation for BD LSRFortessa X-20. Version 2.2 - September 2024
EuroFlow SOP for instrument set-up and compensation for Navios. Version 1.5.1 - October 2019
List of reference and alternative antibodies for hematological malignancies panels. Version 1.13 - November 2023
Reference values for PB leukocyte populations in percentages (EuroFlow PIDOT). Version 1.2 - December 2024
Reference values for PB leukocyte populations in absolute numbers of cells (EuroFlow PIDOT). Version 1.1 - July 2024
The above documents can be accessed after creating a EuroFlow account and upon login at app.euroflow.org/login
Videos
Educational video: EuroFlow SOP for instrument setup and compensation (for BD LSR II and BD FACS Canto)
euroflow.org
Publications
Neirinck et al., The EuroFlow PIDOT external quality assurance scheme: enhancing laboratory performance evaluation in immunophenotyping of rare lymphoid immunodeficiencies. Clin Chem Lab Med. 2024 Oct 21.
pubmed.ncbi.nlm.nih.gov
van der Burg et al., The EuroFlow PID Orientation Tube for Flow Cytometric Diagnostic Screening of Primary Immunodeficiencies of the Lymphoid System. Front Immunol. 2019 Mar 4;10:246.
pubmed.ncbi.nlm.nih.gov
van Dongen et al., EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System. Front Immunol. 2019 Jun 13;10:1271.
pubmed.ncbi.nlm.nih.gov
Linskens et al., Improved Standardization of Flow Cytometry Diagnostic Screening of Primary Immunodeficiency by Software-Based Automated Gating. Front Immunol. 2020 Nov 2;11:584646.
pubmed.ncbi.nlm.nih.gov
Neirinck et al., The Euroflow PID Orientation Tube in the diagnostic workup of primary immunodeficiency: Daily practice performance in a tertiary university hospital. Front Immunol. 2022 Sep 13;13:937738.
pubmed.ncbi.nlm.nih.gov
Kalina et al., Quality assessment program for EuroFlow protocols: summary results of four-year (2010-2013) quality assurance rounds. Cytometry A. 2015 Feb;87(2):145-56.
pubmed.ncbi.nlm.nih.gov
Kalina et al., Frequent issues and lessons learned from EuroFlow QA. J Immunol Methods. 2019 Dec;475:112520.
pubmed.ncbi.nlm.nih.gov